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Selenomethylene Locked Nucleic Acid Enables Reversible Hybridization in Response to Redox Changes
Author(s) -
Morihiro Kunihiko,
Kodama Tetsuya,
Moai Yoshihiro,
Veedu Rakesh N.,
Obika Satoshi
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201300555
Subject(s) - nucleic acid , molecular beacon , oligonucleotide , fluorophore , redox , chemistry , computer science , nanotechnology , combinatorial chemistry , computational biology , biophysics , fluorescence , biochemistry , materials science , physics , biology , dna , organic chemistry , quantum mechanics
Locking up selenium : A new conformationally restricted nucleic acid with a 2′,4′‐selenomethylene bridge (SeLNA) can be reversibly converted into its oxidized form (SeOLNA), and the hybridization of a modified oligonucleotide was shown to be dependent on the oxidation state. A SeLNA‐modified molecular‐beacon‐type probe (see scheme; F=fluorophore, Q=quencher) can be used as a sensor for changes in the redox environment.