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Conformational Transitions at an S‐Layer Growing Boundary Resolved by Cryo‐TEM
Author(s) -
Comolli Luis R.,
Siegerist Cristina E.,
Shin SeongHo,
Bertozzi Carolyn,
Regan William,
Zettl Alex,
De Yoreo Jim
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201300543
Subject(s) - computer science , ideal (ethics) , process (computing) , layer (electronics) , boundary layer , boundary (topology) , nanotechnology , information retrieval , materials science , physics , mathematics , philosophy , thermodynamics , mathematical analysis , epistemology , operating system
Snapshots of growth : The use of a single graphene layer as the support for S‐layer self‐assembly of proteins provides an ideal, robust flat surface to image the expanding boundary by flash freezing and cryo‐TEM, capturing the conformational states present at the expanding boundary (see figure). Image alignment and averaging provide a view of the steps leading to subunit recruitment and maturation in the self‐assembly process.