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Single‐Molecule Detection of 5‐Hydroxymethylcytosine in DNA through Chemical Modification and Nanopore Analysis
Author(s) -
Li WenWu,
Gong Lingzhi,
Bayley Hagan
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201300413
Subject(s) - nanopore sequencing , dna , nanopore , 5 hydroxymethylcytosine , computational biology , cytosine , computer science , chemistry , bisulfite , biotinylation , nanotechnology , combinatorial chemistry , dna methylation , dna sequencing , gene , biochemistry , biology , gene expression , materials science
DNA threading the needle : A new method of single‐molecule detection of 5‐hydroxymethylcytosine (5hmC) in DNA has been developed. Selective thiol substitution of 5hmC (giving SMC) in a single‐step, bisulfite‐mediated reaction (see scheme) allows the incorporation of a peptide (yellow sphere) or biotin into DNA. Modified 5hmC bases can be readily distinguished at the single‐molecule level using protein nanopore analysis.