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Integrated Bare Narrow Capillary–Hydrodynamic Chromatographic System for Free‐Solution DNA Separation at the Single‐Molecule Level
Author(s) -
Zhu Zaifang,
Chen Huang,
Wang Wei,
Morgan Aaron,
Gu Congying,
He Chiyang,
Lu Joann J.,
Liu Shaorong
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201300208
Subject(s) - injector , analyte , capillary action , microfluidics , chromatography , coupling (piping) , dna , chemistry , range (aeronautics) , volume (thermodynamics) , computer science , analytical chemistry (journal) , nanotechnology , materials science , physics , thermodynamics , biochemistry , metallurgy , composite material
Turn the volume down : Coupling a high‐pressure electroosmotic pump (EOP) and a microfabricated chip‐injector with a bare narrow capillary–hydrodynamic chromatographic system (BaNC‐HDC), enables samples to be injected at low‐picoliter volumes, analytes to be eluted at picoliters per minute, and a wide size range of DNA fragments to be resolved (see picture) rapidly in free solution at the single‐molecule level.