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Molecular Imaging of Cancer Cells Using a Bacteriophage‐Based 129 Xe NMR Biosensor
Author(s) -
Palaniappan Krishnan K.,
Ramirez R. Matthew,
Bajaj Vikram S.,
Wemmer David E.,
Pines Alexander,
Francis Matthew B.
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201300170
Subject(s) - epidermal growth factor receptor , nuclear magnetic resonance spectroscopy , xenon , biosensor , chemistry , bacteriophage , molecule , cancer cell , nmr spectra database , nuclear magnetic resonance , biophysics , receptor , spectral line , cancer , biochemistry , stereochemistry , biology , physics , escherichia coli , organic chemistry , astronomy , gene , genetics
NMR imaging : Filamentous fd bacteriophage expressing antibodies recognizing the epidermal growth factor receptor (EGFR) were modified to incorporate cage‐like xenon‐binding molecules (CryA). The resulting contrast agent was shown to bind to an EGFR‐positive cell line and detected by hyperpolarized 129 Xe NMR spectroscopy using chemical exchange saturation transfer (hyperCEST, see picture).