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Steady‐State Continuous‐Flow Purification by Electrophoresis
Author(s) -
Agostino Fletcher J.,
Cherney Leonid T.,
Galievsky Victor,
Krylov Sergey N.
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201300104
Subject(s) - free flow electrophoresis , continuous flow , flow (mathematics) , electrolyte , electrophoresis , bubble , steady state (chemistry) , chromatography , separation (statistics) , channel (broadcasting) , electrode , mechanics , chemistry , materials science , computer science , physics , electrical engineering , engineering , polyacrylamide gel electrophoresis , gel electrophoresis of proteins , enzyme , biochemistry , machine learning
Bubble and sweep : Free‐flow electrophoresis (FFE) could allow continuous‐flow purification of products from continuous‐flow microsynthesis. However, gas bubbles distort the separation. An open‐electrolyte FFE system avoids this problem. Electrodes placed in chimneys open to the atmosphere mean bubbles are vented directly from the system and cannot enter the separation channel (see picture).