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Red Fluorescent Probe for Monitoring the Dynamics of Cytoplasmic Calcium Ions
Author(s) -
Egawa Takahiro,
Hirabayashi Kazuhisa,
Koide Yuichiro,
Kobayashi Chiaki,
Takahashi Naoya,
Mineno Tomoko,
Terai Takuya,
Ueno Tasuku,
Komatsu Toru,
Ikegaya Yuji,
Matsuki Norio,
Nagano Tetsuo,
Hanaoka Kenjiro
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201210279
Subject(s) - fluorescence , cytoplasm , computer science , nanotechnology , chemistry , biophysics , second messenger system , live cell imaging , calcium , biochemistry , biology , physics , materials science , cell , optics , intracellular , organic chemistry
We see red (and yellow and green) : Probe 1 was developed for the visualization of cytoplasmic Ca 2+ , a pivotal second messenger in many biological responses. The new probe is suitable for multicolor imaging for the simultaneous detection of metal ions or proteins and is superior to the existing red fluorescent probe Rhod‐2 for the monitoring of cytoplasmic Ca 2+ oscillation in cultured cells (see fluorescence images of cells with 1 (top) and Rhod‐2 (bottom)).