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Nitroxyl Radicals for Studying Electron Transfer
Author(s) -
Eliash Tamar,
Barbon Antonio,
Brustolon Marina,
Sheves Mordechai,
Bilkis Itzhak,
Weiner Lev
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201210207
Subject(s) - electron paramagnetic resonance , bacteriorhodopsin , chemistry , nitroxyl , radical , electron transfer , ruthenium , combinatorial chemistry , photochemistry , computer science , membrane , organic chemistry , biochemistry , physics , nuclear magnetic resonance , catalysis
Electron‐transfer (ET) rates are measured by use of time‐resolved EPR spectroscopy, involving photooxidation of nitroxyl radicals by a ruthenium bipyridyl complex. This permits acquisition of the fundamental characteristics of ET in solution. The method was used on two spin‐labeled derivatives of bacteriorhodopsin, and is applicable to proteins, nucleic acids, and biological membranes.