Premium
Probing Local Backbone Geometries in Intrinsically Disordered Proteins by Cross‐Correlated NMR Relaxation
Author(s) -
Stanek Jan,
Saxena Saurabh,
Geist Leonhard,
Konrat Robert,
Koźmiński Wiktor
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201210005
Subject(s) - intrinsically disordered proteins , relaxation (psychology) , chemical physics , anisotropy , population , chemistry , dipole , nuclear magnetic resonance , materials science , crystallography , physics , optics , organic chemistry , psychology , social psychology , demography , sociology
An ultra‐high‐resolution NMR experiment for the measurement of intraresidue 1 H( i )– 15 N( i )– 13 C′( i ) dipolar–chemical shift anisotropy relaxation interference is employed to extract information about local backbone geometries in intrinsically disordered proteins. The study of tumor suppressor BASP1 revealed a population shift of β‐turn geometries at low pH conditions and a compaction of the BASP1 structural ensemble.