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Monovalent Streptavidin that Senses Oligonucleotides
Author(s) -
Taylor Steven K.,
Wang Jingxian,
Kostic Natasa,
Stojanovic Milan N.
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201209948
Subject(s) - streptavidin , oligonucleotide , biotin , biotinylation , chemistry , dissociation (chemistry) , biophysics , combinatorial chemistry , biochemistry , biology , dna
Only one place available : Monovalent streptavidin is prepared in a one‐step process based on a trisbiotinylated oligonucleotide that blocks three of streptavidin's four biotin‐binding sites. The complex is highly sensitive to single‐base differences: perfectly matched oligonucleotides trigger dissociation of the biotin–streptavidin interaction at higher rates than strands with single‐base mismatches.