Premium
Synthesis of Autophagosomal Marker Protein LC3‐II under Detergent‐Free Conditions
Author(s) -
Huang YiChao,
Li YiMing,
Chen Yang,
Pan Man,
Li YiTong,
Yu Li,
Guo QingXiang,
Liu Lei
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201209523
Subject(s) - computer science , scheme (mathematics) , phosphatidylethanolamine , computational biology , chemistry , biochemistry , biology , mathematics , mathematical analysis , phospholipid , membrane , phosphatidylcholine
Just add oil : A new detergent‐free method was developed to synthesize lipidated proteins using a light‐activatable solubilizing side chain (in dashed circle, see scheme) to assist the ligation of the lipopeptides. This method allows the efficient preparation of a phosphatidylethanolamine‐conjugated autophagosomal marker protein (LC3‐II) as well as labeled derivatives of LC3‐II, which can be used to study autophagy regulation.