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Discovery of Hepatitis C Virus NS3 Helicase Inhibitors by a Multiplexed, High‐Throughput Helicase Activity Assay Based on Graphene Oxide
Author(s) -
Jang Hongje,
Ryoo SooRyoon,
Kim YoungKwan,
Yoon Soojin,
Kim Henna,
Han Sang Woo,
Choi ByongSeok,
Kim DongEun,
Min DalHee
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201209222
Subject(s) - virology , ribavirin , hepatitis c virus , boceprevir , ns5b , ns3 , hepatitis c , ns5a , helicase , medicine , virus , biology , hepacivirus , rna , gene , biochemistry
A GO‐to solution : A simple graphene oxide (GO)‐based assay to screen for selective inhibitors of a hepatitis C virus (HCV) helicase along with inhibitors of a severe acute respiratory syndrome coronavirus (SARS CoV) helicase was tested (see scheme). A single screen found five inhibitors highly selective for the HCV helicase orthologous to the SARS CoV helicase. Some of these hits were validated using the same GO‐based assay.