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Expression of Fluorescent Cyclotides using Protein Trans‐Splicing for Easy Monitoring of Cyclotide–Protein Interactions
Author(s) -
Jagadish Krishnappa,
Borra Radhika,
Lacey Vanessa,
Majumder Subhabrata,
Shekhtman Alexander,
Wang Lei,
Camarero Julio A.
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201209219
Subject(s) - intein , green fluorescent protein , chemistry , rna splicing , fluorescence , amino acid , biochemistry , protein expression , biology , computational biology , rna , gene , physics , quantum mechanics
Going in circles : Expression of cyclotides containing non‐natural amino acids (see scheme; gray sphere) inside live bacterial cells was accomplished using a highly efficient split intein in combination with nonsense codon suppressor tRNA technology. Cyclotides containing p ‐azidophenylalanine can be expressed in live bacterial cells and easily labeled using copper‐free click chemistry to monitor the cyclotide–protein interactions.