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A Protein‐Interaction Array Inside a Living Cell
Author(s) -
Gandor Silke,
Reisewitz Stephanie,
Venkatachalapathy Muthukumaran,
Arrabito Giuseppe,
Reibner Martina,
Schröder Hendrik,
Ruf Katharina,
Niemeyer Christof M.,
Bastiaens Philippe I. H.,
Dehmelt Leif
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201209127
Subject(s) - förster resonance energy transfer , protein–protein interaction , biological system , biophysics , cell , immunoprecipitation , living cell , interaction network , biology , chemistry , microbiology and biotechnology , fluorescence , antibody , physics , biochemistry , genetics , gene , quantum mechanics
Taking the bait : Protein‐interaction arrays were generated in living cells by the interaction of bait‐presenting artificial receptor constructs (bait‐PARCs) with micrometer‐scaled antibody surface patterns (see figure). This method was applied to simultaneously monitor the interaction kinetics of a prey protein with two distinct bait proteins in individual living cells.