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Two‐Color Glycan Labeling of Live Cells by a Combination of Diels–Alder and Click Chemistry
Author(s) -
Niederwieser Andrea,
Späte AnneKatrin,
Nguyen Long Duc,
Jüngst Christian,
Reutter Werner,
Wittmann Valentin
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201208991
Subject(s) - click chemistry , cycloaddition , glycan , azide , chemistry , diels–alder reaction , alkyne , visualization , combinatorial chemistry , organic chemistry , computer science , biochemistry , catalysis , glycoprotein , artificial intelligence
One is not enough : Terminal alkenes are used as chemical reporters and ligation partners for 1,2,4,5‐tetrazines in a Diels–Alder reaction with inverse electron demand (DARinv). Combination with strain‐promoted azide–alkyne cycloaddition (SPAAC) allows the visualization of two different glycan structures in one experiment.