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Liquid AP‐UV‐MALDI Enables Stable Ion Yields of Multiply Charged Peptide and Protein Ions for Sensitive Analysis by Mass Spectrometry
Author(s) -
Cramer Rainer,
Pirkl Alexander,
Hillenkamp Franz,
Dreisewerd Klaus
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201208628
Subject(s) - mass spectrometry , chemistry , electron transfer dissociation , ion , tandem mass spectrometry , maldi imaging , ionization , dissociation (chemistry) , fragmentation (computing) , analytical chemistry (journal) , mass spectrum , collision induced dissociation , electrospray ionization , top down proteomics , matrix assisted laser desorption/ionization , protein mass spectrometry , biomolecule , electrospray , desorption , chromatography , adsorption , biochemistry , organic chemistry , computer science , operating system
All charged up : High and prolonged yields of multiply charged peptide and protein ions can be formed in MALDI mass spectrometry using liquid UV‐MALDI matrices and a heated ion‐transfer tube. The key features are low laser energies of 1–10 μJ, resulting in fluences of less than 200 to 2000 J m −2 and low sample ablation, high sensitivity, and continuous ion generation over tens of thousands of laser shots.