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Polymer Nanofiber‐Embedded Microchips for Detection, Isolation, and Molecular Analysis of Single Circulating Melanoma Cells
Author(s) -
Hou Shuang,
Zhao Libo,
Shen Qinglin,
Yu Juehua,
Ng Charles,
Kong Xiangju,
Wu Dongxia,
Song Min,
Shi Xiaohong,
Xu Xiaochun,
OuYang WeiHan,
He Rongxian,
Zhao XingZhong,
Lee Tom,
Brunicardi F. Charles,
Garcia Mitch André,
Ribas Antoni,
Lo Roger S.,
Tseng HsianRong
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201208452
Subject(s) - laser capture microdissection , computer science , nanofiber , computational biology , microdissection , melanoma , nanotechnology , information retrieval , chemistry , materials science , biology , cancer research , gene , biochemistry , gene expression
Confined to one cell : A method to detect and isolate single circulating melanoma cells (CMCs; see figure) has been produced by integrating a polymer‐nanofiber‐embedded nanovelcro cell‐affinity assay with a laser microdissection (LMD) technique. This method is able to separate CMCs from normal white blood cells (WBCs) and sequence individual cells for a specific mutation related to cancer progression, allowing for more personalized cancer therapy.