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Combinatorial Nucleoside‐Deletion‐Scanning Mutagenesis of Functional DNA
Author(s) -
Samanta Biswajit,
Höbartner Claudia
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201208103
Subject(s) - dna , mutagenesis , computer science , computational biology , nucleic acid , nucleoside , nucleotide , information retrieval , genetics , mutation , biology , gene
Compact and informative : Individual nucleosides are statistically mutated by replacement with a non‐nucleosidic spacer unit Δ that encodes the “deletion” in a single DNA library. This efficient mutagenesis approach enables minimization of functional DNA, identifying essential nucleotides, as demonstrated for two nucleic‐acid ligating deoxyribozymes.