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Novel Activity‐Based Probes for Broad‐Spectrum Profiling of Retaining β‐Exoglucosidases In Situ and In Vivo
Author(s) -
Kallemeijn Wouter W.,
Li KahYee,
Witte Martin D.,
Marques André R. A.,
Aten Jan,
Scheij Saskia,
Jiang Jianbing,
Willems Lianne I.,
VoornBrouwer Tineke M.,
van Roomen Cindy P. A. A.,
Ottenhoff Roelof,
Boot Rolf G.,
van den Elst Hans,
Walvoort Marthe T. C.,
Florea Bogdan I.,
Codée Jeroen D. C.,
van der Marel Gijsbert A.,
Aerts Johannes M. F. G.,
Overkleeft Herman S.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201207771
Subject(s) - in situ , aziridine , in vivo , broad spectrum , chemistry , glycoside hydrolase , computational biology , stereochemistry , biochemistry , biophysics , biology , combinatorial chemistry , enzyme , genetics , organic chemistry , ring (chemistry)
A high‐end label : Cyclophellitol aziridine‐type activity‐based probes allow for ultra‐sensitive visualization of mammalian β‐glucosidases (GBA1, GBA2, GBA3, and LPH) as well as several non‐mammalian β‐glucosidases (see picture). These probes offer new ways to study β‐exoglucosidases, and configurational isomers of the cyclophellitol aziridine core may give activity‐based probes targeting other retaining glycosidase families.