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A Ratiometric Fluorescent Probe for Rapid Detection of Hydrogen Sulfide in Mitochondria
Author(s) -
Chen Yuncong,
Zhu Chengcheng,
Yang Zhenghao,
Chen Junjie,
He Yafeng,
Jiao Yang,
He Weijiang,
Qiu Lin,
Cen Jiajie,
Guo Zijian
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201207701
Subject(s) - hydrogen sulfide , fluorescence , sulfide , mitochondrion , chemistry , photochemistry , biophysics , biochemistry , biology , organic chemistry , physics , optics , sulfur
Quick : An exogenously induced quick increase of the H 2 S concentration (80 s) in MCF‐7 cells can be visualized by ratiometric imaging using a new probe (CouMC) that can target mitochondria. CouMC was constructed by combining merocyanine and coumarin fluorophores. The selective nucleophilic addition of HS − to the merocyanine derivative at neutral pH is crucial for the rapid H 2 S detection.