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Rewiring Translation for Elongation Factor Tu‐Dependent Selenocysteine Incorporation
Author(s) -
Aldag Caroline,
Bröcker Markus J.,
Hohn Michael J.,
Prat Laure,
Hammond Gifty,
Plummer Abigail,
Söll Dieter
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201207567
Subject(s) - selenocysteine , ribosome , transfer rna , elongation factor , ef tu , translation (biology) , chemistry , biochemistry , computational biology , computer science , biology , rna , enzyme , gene , cysteine , messenger rna
Enjoying UTu in concert : A synthetic tRNA (tRNA UTu ) was used as a substrate for three E. coli proteins: seryl‐tRNA synthetase (SerRS) forming Ser‐tRNA UTu , selenocysteine (Sec) synthase (SelA) generating Sec‐tRNA UTu , and EF‐Tu for Sec‐tRNA UTu transport to the ribosome (see scheme). tRNA UTu can be used by the ribosome, thus allowing site‐specific Sec insertion into proteins, including formate dehydrogenase H, selenoglutaredoxin, and glutathione peroxidase.