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Metabolic Differences in Microbial Cell Populations Revealed by Nanophotonic Ionization
Author(s) -
Walker Bennett N.,
Antonakos Cory,
Retterer Scott T.,
Vertes Akos
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201207348
Subject(s) - downregulation and upregulation , saccharomyces cerevisiae , yeast , ionization , computer science , mass spectrometry , intracellular , chemistry , computational biology , information retrieval , biochemistry , biology , gene , chromatography , ion , organic chemistry
Playing (yeast) laser tag : Laser desorption ionization mass spectrometry (LDI‐MS) with nanopost arrays (NAPA, see scheme) was shown to quantitatively analyze trace amounts of intracellular metabolites from single cells of Saccharomyces cerevisiae (baker's yeast). The upregulation and downregulation of metabolites because of external stress (such as oxidative stress) are also observed, even at the single‐cell level.