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A Gel‐Encapsulated Bioreactor System for NMR Studies of Protein–Protein Interactions in Living Mammalian Cells
Author(s) -
Kubo Satoshi,
Nishida Noritaka,
Udagawa Yuko,
Takarada Osamu,
Ogino Shinji,
Shimada Ichio
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201207243
Subject(s) - bioreactor , hela , nuclear magnetic resonance spectroscopy , chemistry , cell , computational biology , biochemistry , computer science , biology , stereochemistry , organic chemistry
Staying alive : The major limitation of in‐cell NMR spectroscopy methods is the occurrence of cell death during the NMR measurement. To overcome this problem, a bioreactor was utilized that can perfuse the cells in the NMR tube, thereby maintaining the conditions for more than 5 h. By using the bioreactor, the binding site of an externally introduced protein for an endogenous molecule in HeLa S3 cells was identified.