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An Acetylene‐Bridged 6,8‐Purine Dimer as a Fluorescent Switch‐On Probe for Parallel G‐Quadruplexes
Author(s) -
Nikan Mehran,
Di Antonio Marco,
Abecassis Keren,
McLuckie Keith,
Balasubramanian Shankar
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201207075
Subject(s) - g quadruplex , nucleic acid , telomere , dna , fluorescence , antiparallel (mathematics) , transcription (linguistics) , small molecule , rna , biomolecule , chemistry , biophysics , biology , computational biology , biochemistry , microbiology and biotechnology , gene , physics , linguistics , philosophy , quantum mechanics , magnetic field
Lighting up : The systematic design and synthesis of a G‐quartet‐inspired fluorescence probe (APD), which is made of two acetylene‐bridged purines, are reported. The APD lights up in the presence of parallel DNA (e.g. c‐MYC) or RNA G‐quadruplexes, while it shows no fluorescence enhancement with double‐stranded DNA, antiparallel or mixed‐type (e.g. h‐Telo) G‐quadruplexes (see picture). The utility of APD in the preferential staining of G‐quadruplexes is also demonstrated.