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Single‐Round, Multiplexed Antibody Mimetic Design through mRNA Display
Author(s) -
Olson C. Anders,
Nie Jeff,
Diep Jonathan,
AlShyoukh Ibrahim,
Takahashi Terry T.,
AlMawsawi Laith Q.,
Bolin Jennifer M.,
Elwell Angela L.,
Swanson Scott,
Stewart Ron,
Thomson James A.,
Soh H. Tom,
Roberts Richard W.,
Sun Ren
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201207005
Subject(s) - multiplexing , continuous flow , power (physics) , computer science , chemistry , engineering , physics , telecommunications , biochemical engineering , quantum mechanics
In a single round : By combining the high‐efficiency enrichment through the continuous‐flow magnetic separation (CFMS) technique with the analytical power of next‐generation sequencing, the generation of antibody mimetics with a single round of mRNA display is made possible. This approach eliminates iterative selection cycles and provides a path to fully automated ligand generation (see picture).