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Enhancement and Modulation of Enzymatic Activity through Higher‐Order Structural Changes of Giant DNA–Protein Multibranch Conjugates
Author(s) -
Rudiuk Sergii,
VenancioMarques Anna,
Baigl Damien
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201206962
Subject(s) - dna , enzyme , conjugate , order (exchange) , biochemistry , gene , chemistry , microbiology and biotechnology , biology , mathematics , mathematical analysis , finance , economics
A giant DNA leap for enzyme‐kind : Giant DNA–protein multibranch conjugates were shown to be composed of a single β‐lactamase enzyme (green, see scheme) conjugated to up to four 48.5 kbp lambda phage DNA branches (blue lines). The conjugation of giant DNA induces a two‐ to fourfold enhancement of the enzymatic activity, which is modulated by changes in the higher‐order DNA structure.