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Absolute and Relative Quantification of Multiplex DNA Assays Based on an Elemental Labeling Strategy
Author(s) -
Han Guojun,
Zhang Sichun,
Xing Zhi,
Zhang Xinrong
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201206903
Subject(s) - multiplex , oligonucleotide , computer science , dna , mass spectrometry , nucleic acid , elemental analysis , chemistry , computational biology , information retrieval , nanotechnology , chromatography , biology , bioinformatics , materials science , biochemistry , organic chemistry
Elements and quantification : A nucleic acid assay has been developed, based on an elemental labeling strategy using magnetic microparticles (MMPs), which provides quantification of multiple DNA targets. Rare‐earth elements, indium, and stable isotopes could be labeled with oligonucleotides serving as DNA probes. Quantitative analysis was then carried out using the designed systems (see picture) and elemental mass spectrometry.