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Simultaneous Measurement of SUMOylation using SNAP/CLIP‐Tag‐Mediated Translation at the Single‐Molecule Level
Author(s) -
Yang Yong,
Zhang Chunyang
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201206695
Subject(s) - sumo protein , snap , scheme (mathematics) , computer science , translation (biology) , service (business) , chemistry , ubiquitin , operating system , mathematics , biochemistry , messenger rna , economics , gene , mathematical analysis , economy
Two opposing SUMO wrestlers : Simultaneous measurement of multiple SUMOylations was achieved at the single‐molecule level by integrating SNAP/CLIP‐tag‐mediated translation with single‐molecule detection (see scheme; SUMO=small ubiquitin‐like modifier). This method gives exceptional sensitivity, and is capable of measuring the SUMOylation of different proteins under various physiological conditions.