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Decrease in RNA Folding Cooperativity by Deliberate Population of Intermediates in RNA G‐Quadruplexes
Author(s) -
Kwok Chun Kit,
Sherlock Madeline E.,
Bevilacqua Philip C.
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201206475
Subject(s) - cooperativity , rna , folding (dsp implementation) , g quadruplex , chemistry , population , biophysics , biology , computational biology , biochemistry , dna , medicine , gene , engineering , electrical engineering , environmental health
Keeping a broad (RNA) perspective : Conventional biochemical detection systems only have a 100‐fold linear response range. The range of potassium concentrations detected by an RNA G‐quadruplex sequence can be broadened by intentionally populating multiple intermediate folding states (see scheme). The folding of the RNA G‐quadruplexes was monitored by both circular dichroism and intrinsic fluorescence spectroscopy.