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Environmental Control of the Calicheamicin Polyketide Synthase Leads to Detection of a Programmed Octaketide and a Proposal for Enediyne Biosynthesis
Author(s) -
Belecki Katherine,
Townsend Craig A.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201206462
Subject(s) - enediyne , calicheamicin , polyketide synthase , biosynthesis , thioesterase , polyketide , chemistry , biochemistry , stereochemistry , enzyme , biology , genetics , antibody
A light in the dark : The fermentation products of the polyketide synthase CalE8 (without its cognate thioesterase) were identified and gave some insight into the elusive early steps of calicheamicin biosynthesis. Fermentation in either the light or dark resulted in different proportions of a new octaketide (see scheme) and led to an updated mechanistic proposal.