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Ligand‐ and pH‐Induced Conformational Changes of RNA Domain Helix 69 Revealed by 2‐Aminopurine Fluorescence
Author(s) -
Sakakibara Yogo,
Abeysirigunawardena Sanjaya C.,
Duc AnneCécile E.,
Dremann Danielle N.,
Chow Christine S.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201206000
Subject(s) - helix (gastropod) , conformational change , rna , biophysics , chemistry , fluorescence , aminoglycoside , ligand (biochemistry) , paromomycin , nucleic acid structure , loop (graph theory) , crystallography , stereochemistry , biochemistry , biology , physics , receptor , gene , antibiotics , ecology , mathematics , quantum mechanics , combinatorics , snail
Loop conformation : The loop of the RNA domain helix 69 (H69) was modified with the fluorescent analogue 2‐aminopurine (2AP), thus showing different conformational states under various conditions. The application of this model RNA reveals the unique impact of aminoglycoside neomycin, which differs from the effects of structurally related compounds paromomycin and gentamicin, on the H69 loop conformation in solution (see picture).