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A Switchable Two‐Photon Membrane Tracer Capable of Imaging Membrane‐Associated Protein Tyrosine Phosphatase Activities
Author(s) -
Li Lin,
Shen Xiaoqin,
Xu QingHua,
Yao Shao Q.
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201205940
Subject(s) - protein tyrosine phosphatase , membrane , phosphatase , fluorescence , chemistry , biophysics , tyrosine , membrane protein , alkaline phosphatase , biochemistry , enzyme , biology , optics , physics
When I look into your cells : A two‐photon dye ( Flu7 ) was developed (see scheme), which strongly fluoresces only upon selective binding to the plasma membrane of mammalian cells. Upon addition of a UV‐ and phosphatase‐controlled quencher ( Q12 ), the system exhibits ON/OFF/ON fluorescence switching and can be used to image membrane‐associated receptor‐like protein tyrosine phosphatase (RPTP) activity.