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DNA Detection Based on Fluorogenic Nanospheres
Author(s) -
Shu Xin,
Liu Yonghui,
Zhu Jin
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201205628
Subject(s) - dna , fluorescence , conjugated system , dna–dna hybridization , chemistry , nanotechnology , hybridization probe , combinatorial chemistry , computational biology , biophysics , biology , biochemistry , materials science , physics , organic chemistry , optics , polymer
High and dry fluorescence : Fluorogenic nanospheres (green, see scheme) are conjugated to DNA (pink) and used to detect target DNA (aqua). Upon addition of N ‐butylmorpholine (droplet), the nanospheres dissolve, releasing fluorophores, and intense blue fluorescence is emitted at the site of DNA hybridization. The separation of DNA hybridization and signal amplification gives high sensitivity (100 zmol) and selectivity.