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Real‐Time Monitoring of Protein Conformational Dynamics in Solution Using Kinetic Capillary Electrophoresis
Author(s) -
Clouthier Christopher M.,
Mironov Gleb G.,
Okhonin Victor,
Berezovski Maxim V.,
Keillor Jeffrey W.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201205575
Subject(s) - electropherogram , capillary electrophoresis , chemistry , conformational isomerism , kinetic energy , kinetics , ligand (biochemistry) , conformational change , chromatography , stereochemistry , molecule , biochemistry , organic chemistry , physics , receptor , quantum mechanics
Conformational analysis : Capillary electrophoresis (CE) allows for the rapid separation of slowly interconverting protein conformers. Kinetic analysis ( k open , k closed , and K d ) of electropherograms in the presence and absence of effector ligands allows the measurement of kinetic and thermodynamic constants associated with conformational changes and ligand binding.