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β 2 ‐Adrenergic Receptor Solutions for Structural Biology Analyzed with Microscale NMR Diffusion Measurements
Author(s) -
Horst Reto,
Stanczak Pawel,
Stevens Raymond C.,
Wüthrich Kurt
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201205474
Subject(s) - microscale chemistry , micelle , diffusion , crystallization , microcoil , chemistry , receptor , materials science , adrenergic receptor , biophysics , analytical chemistry (journal) , chromatography , aqueous solution , thermodynamics , biochemistry , physics , organic chemistry , biology , mathematics education , mathematics , quantum mechanics , electromagnetic coil
Microcoil NMR measurements were performed to determine the final composition of solutions of the β 2 ‐adrenergic receptor (β 2 AR) reconstituted with a detergent and to study the hydrodynamic properties of the detergent micelles containing β 2 AR (see picture). Standards are established for the reproducible preparation of G‐protein‐coupled receptor solutions for crystallization trials and solution NMR studies.