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Observation of Subcellular Metabolite Gradients in Single Cells by Laser Ablation Electrospray Ionization Mass Spectrometry
Author(s) -
Stolee Jessica A.,
Shrestha Bindesh,
Mengistu Getachew,
Vertes Akos
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201205436
Subject(s) - metabolite , compartmentalization (fire protection) , laser capture microdissection , cytoplasm , mass spectrometry , electrospray ionization , computational biology , chemistry , computer science , information retrieval , biology , biochemistry , chromatography , gene expression , gene , enzyme
Getting to the point : Cell microdissection and analysis by the title technique was used to study the compartmentalization of metabolites within subcellular domains. Experiments performed on plant epidermal cells imply that there are significant differences between the metabolite composition in the nucleus and the cytoplasm. This technique is applicable to other cell types and may give insight into the mechanisms of disease and drug delivery.