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A Simple Synthesis of Sugar Nucleoside Diphosphates by Chemical Coupling in Water
Author(s) -
Tanaka Hidenori,
Yoshimura Yayoi,
Jørgensen Malene R.,
CuestaSeijo Jose A.,
Hindsgaul Ole
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201205433
Subject(s) - sugar , nucleotide , nucleoside , simple (philosophy) , chemistry , nucleobase , computer science , reagent , glycosyltransferase , biochemistry , combinatorial chemistry , organic chemistry , dna , philosophy , gene , epistemology
Sugar nucleotides made easy : The new reagent “ImIm”, which is formed in situ in water, is shown to activate nucleoside 5′‐phosphates to their imidazolides, these can subsequently couple with sugar‐1‐phosphates; the whole procedure takes place in water. This truly simple method yields a crude product mixture that can be used directly as a source of donors for glycosyltransferase‐mediated oligsaccharide synthesis. In the scheme, B stands for the nucleobases U, A, or G.