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Heterologous Expression and Manipulation of Three Tetracycline Biosynthetic Pathways
Author(s) -
Wang Peng,
Kim Woncheol,
Pickens Lauren B.,
Gao Xue,
Tang Yi
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201205426
Subject(s) - heterologous , tetracycline , heterologous expression , streptomyces , enzyme , gene , biology , biosynthesis , streptomyces aureofaciens , biochemistry , chemistry , genetics , recombinant dna , antibiotics , bacteria
A very accommodating host : Three tetracycline biosynthetic pathways were overexpressed and manipulated in the heterologous host Streptomyces lividans K4‐114. Through the inactivation of various genes and characterization of the resulting biosynthetic intermediates, new tetracycline‐modifying enzymes were identified (see scheme).