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Cooperation of Two Bifunctional Enzymes in the Biosynthesis and Attachment of Deoxysugars of the Antitumor Antibiotic Mithramycin
Author(s) -
Wang Guojun,
Pahari Pallab,
Kharel Madan K.,
Chen Jing,
Zhu Haining,
Van Lanen Steven G.,
Rohr Jürgen
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201205414
Subject(s) - bifunctional , biosynthesis , enzyme , chemistry , phosphofructokinase 2 , biochemistry , sugar , function (biology) , antibiotics , stereochemistry , biology , microbiology and biotechnology , catalysis
Two bifunctional enzymes cooperate in the assembly and the positioning of two sugars, D ‐olivose and D ‐mycarose, of the anticancer antibiotic mithramycin. MtmC finishes the biosynthesis of both sugar building blocks depending on which MtmGIV activity is supported. MtmGIV transfers these two sugars onto two structurally distinct acceptor substrates. The dual function of these enzymes explains two essential but previously unidentified activities.