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Genetically Encoded Cyclopropene Directs Rapid, Photoclick‐Chemistry‐Mediated Protein Labeling in Mammalian Cells
Author(s) -
Yu Zhipeng,
Pan Yanchao,
Wang Zhiyong,
Wang Jiangyun,
Lin Qing
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201205352
Subject(s) - cyclopropene , chemistry , amino acid , biochemistry , computational biology , genetic code , click chemistry , aminoacyl trna synthetase , transfer rna , biology , gene , combinatorial chemistry , rna , organic chemistry
We just click : Genetic incorporation of a cyclopropene amino acid CpK (see scheme) site‐specifically into proteins in E. coli and mammalian cells was achieved using an orthogonal aminoacyl‐tRNA synthetase/tRNA CUA pair (CpKRS/ Mb tRNA CUA ). Cyclopropene exhibited fast reaction kinetics in the photoclick reaction and allowed rapid (ca. 2 min) labeling of proteins.