Genetically Encoded Cyclopropene Directs Rapid, Photoclick‐Chemistry‐Mediated Protein Labeling in Mammalian Cells | Zendy

Yu Zhipeng | Zendy; Pan Yanchao | Zendy; Wang Zhiyong | Zendy; Wang Jiangyun | Zendy; Lin Qing | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Genetically Encoded Cyclopropene Directs Rapid, Photoclick‐Chemistry‐Mediated Protein Labeling in Mammalian Cells

Author(s) -

Yu Zhipeng,

Pan Yanchao,

Wang Zhiyong,

Wang Jiangyun,

Lin Qing

Publication year - 2012

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.201205352

Subject(s) - cyclopropene , chemistry , amino acid , biochemistry , computational biology , genetic code , click chemistry , aminoacyl trna synthetase , transfer rna , biology , gene , combinatorial chemistry , rna , organic chemistry

We just click : Genetic incorporation of a cyclopropene amino acid CpK (see scheme) site‐specifically into proteins in E. coli and mammalian cells was achieved using an orthogonal aminoacyl‐tRNA synthetase/tRNA CUA pair (CpKRS/ Mb tRNA CUA ). Cyclopropene exhibited fast reaction kinetics in the photoclick reaction and allowed rapid (ca. 2 min) labeling of proteins.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research