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Rational Design and Development of Near‐Infrared‐Emitting Firefly Luciferins Available In Vivo
Author(s) -
Kojima Ryosuke,
Takakura Hideo,
Ozawa Takeaki,
Tada Yukio,
Nagano Tetsuo,
Urano Yasuteru
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201205151
Subject(s) - bioluminescence , luciferase , firefly protocol , in vivo , biophysics , chemistry , nanotechnology , materials science , biology , biochemistry , transfection , genetics , zoology , gene
Shine on : A new rational design strategy for near‐infrared‐emitting firefly luciferins that are available in vivo has been developed using intramolecular bioluminescence resonance energy transfer (BRET). The emission wavelength could be freely tuned by the choice of BRET acceptor, and NIR bioluminescence could be detected in living cells and mice without the need for luciferase manipulation.