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Back Cover: Template‐Directed Synthesis in 3′‐ and 5′‐Direction with Reversible Termination (Angew. Chem. Int. Ed. 33/2012)
Author(s) -
Kaiser Andreas,
Spies Sebastian,
Lommel Tanja,
Richert Clemens
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201204827
Subject(s) - cover (algebra) , primer extension , dna synthesis , extension (predicate logic) , int , primer (cosmetics) , dna , replication (statistics) , combinatorial chemistry , solid phase synthesis , sequence (biology) , chemistry , nanotechnology , computer science , stereochemistry , materials science , engineering , biology , base sequence , biochemistry , programming language , virology , organic chemistry , mechanical engineering , peptide
Simultaneous chemical primer extension in both 3′‐ and 5′‐direction is reported by C. Richert et al. in their Communication on page 8299 ff. The use of magnetic beads as a solid support and azidomethyloxycarbonyl moieties as protecting groups provided control over the extension steps directed by a DNA sequence. The method combines features of replication and of automated DNA synthesis.