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Identification and Characterization of the “Super‐Reduced” State of the H‐Cluster in [FeFe] Hydrogenase: A New Building Block for the Catalytic Cycle?
Author(s) -
Adamska Agnieszka,
Silakov Alexey,
Lambertz Camilla,
Rüdiger Olaf,
Happe Thomas,
Reijerse Edward,
Lubitz Wolfgang
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201204800
Subject(s) - hydrogenase , catalysis , catalytic cycle , electron paramagnetic resonance , electrochemistry , combinatorial chemistry , chemistry , active site , characterization (materials science) , block (permutation group theory) , cluster (spacecraft) , computer science , nanotechnology , materials science , electrode , physics , organic chemistry , mathematics , geometry , nuclear magnetic resonance , programming language
Super‐reduced and super‐active : A new redox state in the active site of algal [FeFe] hydrogenases has been identified and characterized by EPR and FTIR spectroscopy. Electrochemical and in vitro essays show that this species is highly active in hydrogen production and suggest that it is a key intermediate in the catalytic cycle of all [FeFe] hydrogenases.