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Sequencing the Sixth Base (5‐Hydroxymethylcytosine): Selective DNA Oxidation Enables Base‐Pair Resolution
Author(s) -
Schüler Peter,
Miller Aubry K.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201204768
Subject(s) - 5 hydroxymethylcytosine , deamination , sodium bisulfite , bisulfite , cytosine , 5 methylcytosine , chemistry , dna , base pair , base (topology) , combinatorial chemistry , biochemistry , dna methylation , organic chemistry , enzyme , gene , mathematical analysis , gene expression , mathematics
Sodium bisulfite promotes both the deformylative deamination of 5‐formylcytosine (5fC) and the decarboxylative deamination of 5‐carboxylcytosine (5caC; see picture). By coupling this bisulfite chemistry with selective oxidations of individual DNA bases, new methods allow, for the first time, the sequencing of 5‐hydroxymethylcytosine (5hmC) with single‐base‐pair resolution.