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Multiplexed In Situ Immunofluorescence Using Dynamic DNA Complexes
Author(s) -
Schweller Ryan M.,
Zimak Jan,
Duose Dzifa Y.,
Qutub Amina A.,
Hittelman Walter N.,
Diehl Michael R.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201204304
Subject(s) - dna , immunofluorescence , fluorescence , image (mathematics) , in situ , computational biology , computer science , multiplexing , chemistry , information retrieval , biophysics , nanotechnology , biological system , biology , biochemistry , artificial intelligence , antibody , materials science , optics , physics , genetics , telecommunications , organic chemistry
DNA‐based erasers : The designed strand‐displacement reactions between dynamic DNA probes (carrying fluorescent dyes (★) and quencher domains (•)) and DNA–antibody conjugates generate detachable immunofluoresence‐reporting complexes that can be assembled (first image) and disassembled (second image) reversibly within fixed cells. With this system, different protein targets can be imaged sequentially within the same cells (third image).