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Investigation of RNA–Ligand Interactions by 19 F NMR Spectroscopy Using Fluorinated Probes
Author(s) -
Lombès Thomas,
Moumné Roba,
Larue Valéry,
Prost Elise,
Catala Marjorie,
Lecourt Thomas,
Dardel Frédéric,
Micouin Laurent,
Tisné Carine
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201204083
Subject(s) - riboswitch , swap (finance) , nuclear magnetic resonance spectroscopy , ligand (biochemistry) , chemistry , rna , spectroscopy , visualization , computer science , physics , stereochemistry , biochemistry , data mining , non coding rna , gene , receptor , finance , quantum mechanics , economics
Spy swap : The interaction between an unlabeled RNA and unlabeled ligands (red hexagon) can be monitored by 19 F NMR spectroscopy using small fluorinated diamines (green star) as spy reporters (see scheme). This technique also enables the visualization of the conformational capture of a riboswitch by its ligand.