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Localized Intracellular pH Measurement Using a Ratiometric Photoinduced Electron‐Transfer‐Based Nanosensor
Author(s) -
Marín María J.,
Galindo Francisco,
Thomas Paul,
Russell David A.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201203866
Subject(s) - intracellular , nanosensor , organelle , photoinduced electron transfer , biophysics , confocal microscopy , intracellular ph , fluorescence , chemistry , confocal , electron transfer , spectroscopy , nanotechnology , analytical chemistry (journal) , photochemistry , materials science , biochemistry , biology , microbiology and biotechnology , physics , optics , chromatography , quantum mechanics
Intracellular analysis : Changes in the concentration of hydrogen ions can lead to cellular dysfunction. Thus, quantification of the intracellular pH in localized compartments (see A and B) is important. A pH nanosensor based on photoinduced electron transfer is described that targets acidic organelles. A combination of confocal fluorescence microscopy and spectroscopy is used for localized pH measurements within living cells.