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Convergent Chemical Synthesis of Proteins by Ligation of Peptide Hydrazides
Author(s) -
Fang GeMin,
Wang JiaXing,
Liu Lei
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201203843
Subject(s) - native chemical ligation , peptide , chemical ligation , hydrazide , ligation , residue (chemistry) , chemistry , combinatorial chemistry , peptide synthesis , chemical synthesis , biochemistry , biology , organic chemistry , microbiology and biotechnology , in vitro
Coming together : A generally applicable strategy for convergent chemical synthesis of proteins from multiple peptide segments is developed on the basis of the ligation of peptide hydrazides. The peptide hydrazide intermediates can be made at low cost and the new strategy is used in the synthesis of the 142 residue model protein RpS25 from six peptide segments. PG=protecting group.