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A Selective Fluorescent Probe for Carbon Monoxide Imaging in Living Cells
Author(s) -
Wang Jing,
Karpus Jason,
Zhao Boxuan Simen,
Luo Zheng,
Chen Peng R.,
He Chuan
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201203684
Subject(s) - fluorescence , carbon monoxide , domain (mathematical analysis) , computer science , fluorescent protein , computational biology , nanotechnology , chemistry , green fluorescent protein , combinatorial chemistry , gene , biology , materials science , biochemistry , physics , optics , mathematics , mathematical analysis , catalysis
A genetically encoded fluorescent probe is capable of selectively detecting carbon monoxide inside living cell. The probe, named COSer (CO sensor), consists of a circularly permuted yellow fluorescent protein (cpYFP) inserted into the regulatory domain of the bacterial CO‐sensing protein, CooA, which gives the probe its selective CO‐binding property.