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Bio‐Click Chemistry: Enzymatic Functionalization of PEGylated Capsules for Targeting Applications
Author(s) -
Leung Melissa K. M.,
Hagemeyer Christoph E.,
Johnston Angus P. R.,
Gonzales Catalina,
Kamphuis Marloes M. J.,
Ardipradja Katie,
Such Georgina K.,
Peter Karlheinz,
Caruso Frank
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201203612
Subject(s) - surface modification , pegylation , sortase a , sortase , chemistry , click chemistry , covalent bond , enzyme , bioconjugation , combinatorial chemistry , posttranslational modification , biochemistry , polyethylene glycol , organic chemistry , bacterial protein , gene
All sorted : The enzyme Sortase A was used to catalyze functionalization of PEGylated capsules with an activation‐specific anti‐platelet single‐chain antibody (scFv). This enzymatic method allows fast, covalent, and site‐directed functionalization of delivery vehicles under mild conditions. Activation‐specific anti‐platelet scFv‐coated PEGylated capsules exhibited a high level of selective binding to thrombi, thus suggesting their potential for thrombosis therapy.